(2007) described a polymer capsule conjugated with the humanised A33 mAb (huA33 mAb) formed by a layer-by-layer method, which has shown great promise in the treatment of colon cancer. selectively into cancer cells for colon cancer therapy. With 5-fluorouracil-loaded LC-PLGA NPs, we were able to demonstrate significant increases in the uptake efficiency and cytotoxicity in colon cancer cells that were positive for OCTN2 and ATB0,+. In a 3D spheroid model of tumor growth, LC-PLGA NPs showed increased uptake and enhanced antitumor efficacy. These findings indicate that dual-targeting LC-PLGA NPs to OCTN2 and ATB0,+ has great potential to deliver chemotherapeutic drugs for colon cancer therapy. Dual targeting LC-PLGA NPs to OCTN2 PIK-90 and ATB0,+ can selectively deliver chemotherapeutics to colon cancer cells where both transporters are overexpressed, preventing targeting to normal cells and thus avoiding off-target side effects. for 15?min. The media in 96-well plate was replaced every two days with minimum spheroid disturbance. LC-PLGA NPs penetration into tumor cell spheroid After seeding, the spheroid was allowed to grow for four days. A pre-determined amount of coumarin-6-labeled LC-PLGA NPs was added to a final concentration of 5?g/mL for 2?h. After that, the spheroids were collected and washed with excess of PBS to remove unassociated nanoparticles. They were transferred to slides with 200?L of PBS and analyzed immediately on Nikon confocal microscope (Nikon, Tokyo, Japan) with a 10??objective and 488 laser of Fluorescein isothiocyanate. Z-stack images were obtained at fixed intervals of 10?m from periphery into the spheroid. Image J software was used to quantify the fluorescence intensity of coumarin 6. Anti-tumor efficacy of LC-PLGA NPs in spheroid HCT116 and HT29 spheroids were allowed to grow for 24?h. Spheroids were exposed to 1?g/mL or 10?g/mL 5-FU in either free form or in PLGA NPs or in 10%LC-PLGA NPs (PBS as control group). In all the cases, the spheroids were monitored for morphology and size by Nikon microscope and NIS-elements software 4.20 over the following 10?days. Statistical analysis The data were presented as mean??SD, and Students release test (Figure 1(B)), compared to free 5-FU, 5-FU-loaded LC-PLGA NPs with various surface densities of l-carnitine conjugation (0, 2.5, 5, and 10%) exhibited a much slower but prolonged release of the drug. Coumarin 6 was used as a fluorescence marker to track the uptake process, and release profile of coumarin 6 from nanoparticles is shown in Figure S1, indicating a much lower and prolonged release compared to the coumarin 6 solution. Open in a separate window Figure 1. (A) Particle size and size distribution of PLGA NPs and LC-PLGA NPs, (release profiles of free 5-FU, 5-FU-loaded PLGA NPs and LC-PLGA NPs (cytotoxicity experiments of free 5-FU, 5-FU-loaded PLGA NPs and 5-FU-loaded 10%LC-PLGA NPs were performed in one normal cancer cell line and four colon cancer cell lines; the dose-response curves are presented in Figure 5. In CCD841 cells, 5-FU-loaded PLGA NPs showed less cytotoxicity compared to free 5-FU, but 5-FU-loaded LC-PLGA NPs showed increased cytotoxicity. In the other four cancer cells, nanoparticles always had higher cytotoxicity than free drug, but LC-PLGA NPs had the PIK-90 greatest cytotoxicity effect. Open in a separate window Figure 5. The MTT assay for PIK-90 5-FU, 5-FU-loaded PLGA NPs and 5-FU-loaded LC-PLGA Rabbit Polyclonal to CNTN5 NPs in CCD841 (A); Caco-2 (B); HCT116 (C); HT29 (D); and LS174T (E); (F), the calculated IC50 values. Data are shown as mean??SD, tumors. Accordingly, the 3D spheroids model is increasingly recognised as a suitable tool to evaluate the efficacy of nano-drug carriers for drug delivery (Breslin & ODriscoll, 2013; Wu et?al., 2017). Therefore, we used HCT116 and HT29 spheroids to evaluate the anti-tumor efficiency of LC-PLGA NPs as a delivery system for 5-FU. With two doses.
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