The pathomechanisms main vitiligo remain unclear, yet may include: Autoimmune, defect in neural rules, over oxidation caused by damage of reduction system, the recent introduction of the formation of addition bodies caused by defect in melanocyte consumption (2). exams, the melanocytes were transplanted to depigmented areas of vitiligo. Repigmentation was observed continually. The outcomes indicated that melanocytes could be selectively proliferated in the moderate. Subsequently, 100 % pure melanocytes with out contamination of fibroblast and keratinocyte were harvested. A total of 34 patients struggling vitiligo pertaining to between three months and 20 years with depigmented area (between 4 cm2and 70% of body surface) were divided into 19 instances of producing stage and 15 instances of stable stage, according to the change of depigmentation. A total of 15 developing instances were positive for the antibody against melanocytes, together with the positive level of 79%. The titers of serum was > 1: 55 in 12 patients in the developing stage, and five developing individuals were 1: 10. Among the 15 stable cases, four were positive, with a positive rate of 27%. Fluorescence of antibody was localized in the cytoplasm of the melanocytes. Autologous melanocytes of vitiligo patients could be selectively proliferated in the moderate. Next, 100 % pure melanocytes with out contamination with fibroblasts and keratinocytes were harvested. A total FD-IN-1 of sixteen vitiligo individuals with 28 depigmented areas (2200 cm2) were cured with FD-IN-1 transplantation of melanocytes. Repigmentation in the transplanted areas appeared since red pigmentation after one month. All the vitiligous areas received transplantation were repigmented considerably with hypo- or hyper-pigmentation after 35 months. After 68 weeks, 87. 5% of lesions showed repigmentation of > 50% in the lesion region. No scarring or additional side-effects occurred. After followup of five years, simply no relapse was observed in transplantation area. Therefore, an immunofluorescence method for the test of FD-IN-1 antibody to melanocytes in the serum of vitiligo patients was established. Transplantation of cultured autologous melanocytes was an effective and safe measure for treatment of vitiligo, particularly for individuals with a large depigmented region. Keywords: vitiligo, melanocyte tradition, antibody against melanocytes, immunofluorescence, autologous transplantation == Advantages == Vitiligo is a common skin disease, with an incidence of 12% (1), in which melanocytes in the diseased area vanish, resulting in depigmentation. The pathomechanisms underlying vitiligo remain not clear, but may include: Autoimmune, defect in neural regulation, over oxidation caused by damage of reduction system, the latest emergence in the formation of inclusion physiques caused by defect in melanocyte absorption (2). It has been identified that components of immune reactions could mediate melanocyte lysis in the serum of incomplete patients with vitiligo (3). IgG in the serum of patients with vitiligo could penetrate cultured melanocytesin vitro, causing apoptosis (4). These findings show that humoral immunity and autoantibodies play an important part in the incident and development of vitiligo. Creating a detection method for anti-melanocyte antibodies, calculating antibody titers in the blood of individuals with vitiligo and watching its affiliation with the disease are crucial pertaining to understanding the pathogenesis of vitiligo, planning restorative interventions and assessing curative effects (4, 5). Since determined by the current study, immunofluorescence testing of anti-melanocyte antibodies, melanocytes might be culturedin vitroand pure cells harvested. A benefit of this method is that there FD-IN-1 are enough FD-IN-1 uniform melanocytes for discovering the antibodies in individual samples. The main drawbacks are that the success rate of melanocyte culture is usually low, having a long routine and high price, and it is easy to develop contaminants of pores and skin fibroblasts and keratinocytes. On the basis of successfully cultured melanocytes, an immunofluorescence assay of anti-melanocyte antibodies might be conducted using the serum of vitiligo individuals. The present research demonstrated that vitiligo patient serum may consist of anti-melanocyte antibodies, and the Spp1 antibody positive fluorescent coloration is located in the cytoplasm of melanocytes. The titer and the positive rate are associated with the development and balance of disease. Certain medicines and physiotherapy, such as steroids and UVA, have poor efficacy pertaining to treating vitiligo. Autologous pores and skin grafting to supplement melanocytes has been suggested to be a highly effective treatment pertaining to vitiligo (6, 7). However , it is difficult to treat cases concerning large lesions due to the limited availability of graft skin. Causes of allogeneic melanocytes are more abounding; however , there might be a rejection reaction. There are only individual exploratory reviews, with poor clinical outcomes (8). Reviews on transplantation of autologous melanocytes culturedin vitroto deal with vitiligo have already been published (9, 10). A benefit of this strategy is that enough melanocytes can be harvested for any large area of the transplant. The main drawbacks are that the success rate of tradition is low, with a lengthy treatment routine, and that a white boundary area between transplant and the normal pores and skin area might develop. Autologous melanocyte transplantation is suitable for individuals in with stable phase vitiligo, while individuals in advancement stage might be vulnerable to relapse (11). In the present study, the levels of each individuals own defense fluorescent antibody were at first detected, and transplantation.